Differential Expression Pattern of ANS Genes in Different Color Radish(Raphanus sativus L.)

doi:10.15933/j.cnki.1004-3268.2019.12.014

Abstract

Abstract: To explore the relationship between expression level of ANS gene and pigment content in different color radish,sixteen radish varieties with different color fleshy roots were collected as materials and their expression levels of ANS gene were detected from cDNA samples using real-time PCR technology,as well as their pigments content were determined.Subsequently,the relationship between expression level of ANS gene and the content of pigment was explored.The results showed that,the content of pigment(F=114.294 0,P=0.000 1) and expression level of ANS gene(F=95.984 0,P=0.000 1)were both significantly changed in different color radish respectively. Especially it was found that the content of pigment and expression level of ANS gene were both significantly higher in red radish(23.80‰,6.439 0)than those in white radish(0.10‰,0.126 8); In addition,the correlation analysis between expression level of ANS gene and content of pigment in different color radish was conducted,and it was found that their correlation reached extremely significant level and correlation coefficient was 0.83.it was inferred that ANS gene might be one of key regulators in pigment synthesis of radish.

Key words: Radish(Raphanus sativus L.), Pigment content, ANS gene, Correlation

摘要： 为了解不同肉质颜色萝卜ANS基因的表达量与萝卜色素含量的关系，以16份不同肉质颜色萝卜为材料，采用荧光定量PCR法检测cDNA样本中的ANS基因表达量，同时测定萝卜肉质根的色素含量，分析萝卜色素含量与ANS基因表达量的相关关系。结果表明，16份不同肉质颜色萝卜间色素含量存在极显著差异（F=114.294 0，P=0.000 1）。其中，红皮红心萝卜肉质根色素含量（23.80‰）显著高于白皮白心萝卜色素含量（0.10‰）；16份不同肉质颜色萝卜ANS基因表达量也存在极显著差异（F=95.984 0，P=0.000 1），其中，红皮红心萝卜ANS基因表达量最高（6.439 0），白皮白心萝卜ANS基因表达量最低（0.126 8）。相关分析结果表明，萝卜ANS基因表达量与萝卜色素含量的相关系数为0.83，且达到极显著水平。

关键词: 萝卜, 色素含量, ANS基因, 相关性

CLC Number:

S631.1

LIU Hongfang, CHEN Fabo, LI Wenbo, FANG Ping. Differential Expression Pattern of ANS Genes in Different Color Radish(Raphanus sativus L.)[J]. Journal of Henan Agricultural Sciences, 2019, 48(12): 98-102.

刘红芳, 陈发波, 李文博, 方平. 不同肉质颜色萝卜ANS基因表达差异分析[J]. 河南农业科学, 2019, 48(12): 98-102.

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