河南农业科学 ›› 2020, Vol. 49 ›› Issue (9): 153-158.DOI: 10.15933/j.cnki.1004-3268.2020.09.019

• 畜牧·兽医 • 上一篇    下一篇

猪肺炎支原体HNMhy1株免疫原性基因的鉴定与分析

徐引弟,张青娴,王治方,焦文强,李海利,朱文豪,王克领   

  1. (河南省农业科学院畜牧兽医研究所/河南省畜禽繁育与营养调控重点实验室,河南 郑州 450002)
  • 收稿日期:2020-02-16 出版日期:2020-09-15 发布日期:2020-09-15
  • 作者简介:徐引弟(1974-),女,湖北浠水人,副研究员,博士,主要从事动物病原微生物研究。E-mail:445177674@qq.com
  • 基金资助:
    河南省财政预算项目(20188110);河南省农业科学院自主创新基金项目(2017ZC49)

Identification and Analysis of Immunogenic Genes of Mycoplasma hyopneumoniae HNMhy1 Strain

XU Yindi,ZHANG Qingxian,WANG Zhifang,JIAO Wenqiang,LI Haili,ZHU Wenhao,WANG Keling   

  1. (Institute of Animal Husbandry and Veterinary Research,Henan Academy of Agricultural Sciences/Henan Key Laboratory of Farm Animal Breeding and Nutritional Regulation,Zhengzhou 450002,China)
  • Received:2020-02-16 Published:2020-09-15 Online:2020-09-15

摘要: 为了研究猪肺炎支原体的致病机制,对其遗传变异及分子流行病学进行研究,以防控猪支原体肺炎。对分离的猪肺炎支原体河南株HNMhy1的主要免疫原性基因P36、P46、P97、P65、P110进行扩增测序,与GenBank中已公布的猪肺炎支原体菌株的序列进行比对,同源性均在98%以上,部分毒株同源性高达100%。将HNMhy1株的P36基因的核苷酸序列与GenBank中其他菌株基因序列进行比对,同源性均在98%以上;系统进化树显示,河南分离株HNMhy1与湖南株HN13的进化关系最近。以上结果表明,河南分离株HNMhy1主要免疫原性基因高度保守,在遗传演化中未发生太大变化。

关键词: 猪肺炎支原体, HNMhy1株, 免疫原性基因, 同源性分析, 遗传变异

Abstract: In order to further study the pathogenic mechanism of Mycoplasma hyopneumoniae(Mhp),the genetic variation and molecular epidemiology of Mhp were studied,so as to prevent and control Mhp.The main immunogenic genes P36,P46,P97,P65,P110 of the isolated Henan strain HNMhy1 of Mhp were amplified,sequenced and compared to existing sequences in GenBank.The sequences homology with the published Mhp strains were above 98%.Some strains had homology as high as 100%.The nucleotide sequence of P36 gene of HNMhy1 strain was compared with the gene sequence of other strains in GenBank,and the homology was above 98%. Phylogenetic tree displayed that the evolutionary relationship between the isolate HNMhy1 and Hunan strain HN13 was closet.The above results showed that these main immunogenic genes were highly conserved and had no much change in genetic evolution.

Key words: Mycoplasma hyopneumoniae, HNMhy1 strain, Immunogenic gene, Homology analysis, genetic variation

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