河南农业科学 ›› 2020, Vol. 49 ›› Issue (9): 143-152.DOI: 10.15933/j.cnki.1004-3268.2020.09.018

• 畜牧·兽医 • 上一篇    下一篇

不同教槽料对断奶仔猪肠道黏膜免疫相关基因表达的影响

冯宝宝1,赵莹2,马晓宇1,宁丽丽1,赵国琦1,霍永久1   

  1. 1.扬州大学 动物科学与技术学院,江苏 扬州 225009;2.上海新邦生物科技有限公司,上海 200000)
  • 收稿日期:2020-03-26 出版日期:2020-09-15 发布日期:2020-09-15
  • 通讯作者: 霍永久(1971-),男,江苏东海人,副教授,博士,主要从事猪营养、生产与环境研究。E-mail:yjhuo@yzu.edu.cn
  • 作者简介:冯宝宝(1994-),女,河北沧州人,在读硕士研究生,研究方向:猪营养、生产与环境。E-mail:fengbaobao1994@163.com
  • 基金资助:
    江苏省苏北科技专项(SZ-HA201803)

Effects of Different Creep Diet on Expression of Immune Related Genes in Intestinal Mucosa of Weaned Piglets

FENG Baobao1,ZHAO Ying2,MA Xiaoyu1,NING Lili1,ZHAO Guoqi1,HUO Yongjiu1   

  1. (1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.Shanghai Xinbang Biological Technology Co.,Ltd.,Shanghai 200000,China)
  • Received:2020-03-26 Published:2020-09-15 Online:2020-09-15

摘要: 为探讨不同教槽料对断奶仔猪肠道黏膜免疫相关基因表达的影响,选取90头21日龄体质量[(6.85±0.25)kg)]相近的杜洛克×长白×大约克断奶仔猪,随机分为3组。对照组(CON)饲喂基础饲粮,优质蛋白源组(HP)在基础饲粮中添加优质蛋白原料(基础饲粮中的豆粕蛋白原料用发酵酶解豆粕、酵母提取物和小麦水解蛋白粉代替),优质蛋白源+肠道保护包组(HPP)在基础原料中添加优质蛋白原料和肠道保护包(主要成分为复合酸化剂、植物精油、复合益生菌组合)。试验期14 d。采用实时荧光定量PCR(qRT-PCR)方法检测仔猪饲喂不同教槽料后,十二指肠、空肠、回肠、结肠黏膜TNF-α、IL-1β、TGF-β、IL-10的基因表达量,回肠和结肠黏膜紧密连接蛋白Occludin、Claudin-1、ZO-1的基因表达情况。结果显示,与CON组相比,仔猪断奶3 d时,HPP组十二指肠IL-1β及回肠IL-1β、Occludin、Claudin-1、ZO-1的基因表达量均显著升高(P<0.05);仔猪断奶7 d时,HP组和HPP组空肠、回肠TNF-α、IL-10的基因表达量升高(P<0.05),并且回肠Occludin、Claudin-1、ZO-1的基因表达量显著升高(P<0.05);仔猪断奶14 d时,HPP组空肠TNF-α、IL-1β、TGF-β、IL-10和回肠TGF-β的基因表达量显著升高(P<0.05)。可见,断奶仔猪饲喂新型教槽料能够上调肠道黏膜免疫因子相关基因的表达量,增强早期断奶仔猪回肠和结肠黏膜中紧密连接蛋白相关基因的表达量,降低断奶应激对仔猪肠道造成的损害。

关键词: 断奶仔猪, 教槽料, 优质蛋白源, 肠道保护包, 肠道黏膜细胞因子, 紧密连接蛋白

Abstract: In order to reveal the effect of different creep diet on the expression of immune related genes in intestinal mucosa of weaned piglets,ninety 21 day-old(Duroc×Landrace ×Yorkshire) weaned piglets with similar body weight[(6.85±0.25) kg] were randomly divided into three groups.The control group(CON) was fed with basal creep diet;high-quality protein source group(HP):the soybean meal in the basic diet were replaced by fermented and enzymolysis soybean meal,yeast extract and wheat hydrolyzed protein powder;high-quality protein source+intestinal protection package group(HPP):high-quality protein source and intestinal protective package in the basic diet(intestinal protective package mainly consists of compound acidifier,plant essential oil and compound probiotics).The experimental period was 14 d.The expression levels of TNF-α,IL-1β,TGF-β and IL-10 genes in duodenum,jejunum,ileum and colon,and the expression levels of Occludin,Claudin-1 and ZO-1 genes in ileum and colon were analyzed by qRT-PCR.The results showed that,compared with CON group,weaning for 3 days,the HPP group significantly increased the expression levels of IL-1β gene in duodenum and IL-1β,Occludin,Claudin-1,ZO-1 genes in ileum. Weaning for 7 days,the HP group and HPP group significantly increased the expression levels of TNF-α and IL-10 genes in jejunum and ileum(P<0.05),and Occludin,Claudin-1,ZO-1 genes in ileum(P<0.05).Weaning for 14 days,HPP group significantly increased the expression levels of TNF-α,IL-1β,TGF-β,IL-10 genes in jejunum and TGF-β genes in ileum(P<0.05).In conclusion,weaned piglets fed with new creep diet can increase the expression levels of intestinal mucosal immune factors,tight junction proteins related genes in the ileum and colonic mucosa during early weaning piglets,and reduce weaning stress damage to the intestine.

Key words: Weaned piglets, reep diet, High-quality protein source;Intestinal protective package;Intestinal mucosal cytokines;Tight junction protein

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