河南农业科学 ›› 2020, Vol. 49 ›› Issue (9): 81-87.DOI: 10.15933/j.cnki.1004-3268.2020.09.010

• 植物保护 • 上一篇    下一篇

稻瘟病菌对咪鲜胺和三环唑的敏感性及两者的协同作用

刘世江1,2,赵琪君1,2,丁怡1,2,李荣玉1,2,3,李明1,2,3,吴小毛1,2,3   

  1. 1.贵州大学 作物保护研究所,贵州 贵阳 550025;2.贵州大学 猕猴桃工程技术研究中心,贵州 贵阳 550025;3.贵州省山地农业病虫害重点实验室,贵州 贵阳 550025)
  • 收稿日期:2020-02-20 出版日期:2020-09-15 发布日期:2020-09-15
  • 通讯作者: 李荣玉(1984-)男,河南淮阳人,副教授,博士,主要从事农药毒理学与分子生物学及农产品质量安全研究。E-mail:lirongyu0328@126.com
  • 作者简介:刘世江(1995-),男,贵州麻江人,在读硕士研究生,研究方向:农产品质量安全。E-mail:942216633@qq.com
  • 基金资助:
    国家自然科学基金项目(31701816);贵州省科学技术基金计划项目(黔科合基础[2017]1083);国家重点研发计划项目(2016YFD0200500)

Sensitivity of Magnaporthe oryzae to Prochloraz and Tricyclazole and The Synergistic Effect of Two Agents

LIU Shijiang1,2,ZHAO Qijun1,2,DING Yi1,2,LI Rongyu1,2,3,LI Ming1,2,3,WU Xiaomao1,2,3   

  1. (1.Institute of Crop Protection,Guizhou University,Guiyang 550025,China; 2.Engineering and Technology Research Center
    of Kiwifruit,Guizhou University,Guiyang 550025,China; 3.Provincial Key Laboratory for Agricultural Pest Management in Mountainous Region,Guiyang 550025,China)
  • Received:2020-02-20 Published:2020-09-15 Online:2020-09-15

摘要: 为明确稻瘟病菌对咪鲜胺和三环唑的敏感性及两者的协同作用,从贵州、湖北、河南、湖南省稻区采集分离得到103株稻瘟病菌菌株,采用菌丝生长速率法测定稻瘟病菌菌株对咪鲜胺的敏感性,采用水稻苗活体法测定稻瘟病菌对三环唑的敏感性及咪鲜胺和三环唑的协同作用。结果表明,咪鲜胺、三环唑对供试稻瘟病菌菌株的有效抑制中浓度(EC50)值分别为0.004 2~0.056、0.35~9.67 μg/mL,且菌株的敏感性频率分布均近似于正态分布,可将EC50均值0.027、5.24 μg/mL分别作为南方稻区稻瘟病菌对咪鲜胺、三环唑的敏感基线。协同组合筛选结果表明,咪鲜胺与三环唑以有效成分比为4∶20时,增效作用明显,其共毒系数为177.10,EC50值为1.10 μg/mL;该组合在有效成分180 g/hm2剂量下,药后21 d对稻瘟病的田间防效达到86.41%。因此,咪鲜胺、三环唑(有效成分比为4∶20)组合可用于田间防治水稻稻瘟病,推荐剂量为有效成分180 g/hm2

关键词: 稻瘟病菌, 咪鲜胺, 三环唑, 敏感性, 协同作用

Abstract: In order to define the sensitivity sensitivity of Magnaporthe oryzae to prochloraz and tricyclazole and the synergistic effect of two agents,the 103 isolates of M.oryzae were isolated and collected from Guizhou,Hubei,Henan and Hunan Provinces,sensitivity of M.oryzae isolates to prochloraz was determined by mycelial growth rate method,rice seedling method was used to determine the sensitivity of M.oryzae to tricyclazole and the synergistic effect of prochloraz and tricyclazole.The results showed that the EC50 of prochloraz,tricyclazole against M.oryzae were 0.004 2—0.056,0.35—9.67 μg/mL,respectively,and the sensitivity frequency distribution of strain was close to normal distribution.Thus,the average EC50 values 0.027,5.24 μg/mL could be used as basline-sensitivity of M.oryzae to prochloraz,tricyclazole in the southern region of China,respectively.The results of synergistic combination showed that the ratio of obviously effective components of prochloraz to tricyclazole was 4∶20,the co toxicity coefficient(CTC) was 177.10 and EC50 value was 1.10 μg/mL.After 21 d after treatment the field efficacy of this combination(4∶20) of 180 g/ha was 86.41% against rice blast.Thus,the prochloraz and tricyclazole(4∶20)can be applied to control rice blast in field production,and the recommended dosage should be 180 g/ha.

Key words: Magnaporthe oryzae, Prochloraz, Tricyclazole, Sensitivity test, Synergistic effect

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