河南农业科学 ›› 2020, Vol. 49 ›› Issue (6): 120-126.DOI: 10.15933/j.cnki.1004-3268.2020.06.015

• 园艺 • 上一篇    下一篇

基于SCoT标记的猕猴桃种质资源遗传多样性研究

周遇巧1,郭国业1,周索1,杜戈2,李书林2   

  1. (1.南阳师范学院 生命科学与技术学院,河南 南阳 473061;2.河南省西峡猕猴桃研究所,河南 西峡 474573)
  • 收稿日期:2019-12-20 出版日期:2020-06-15 发布日期:2020-06-15
  • 通讯作者: 郭国业(1983-),男,河南南阳人,讲师,博士,主要从事植物系统与分子进化生物学研究。E-mail:guoguoye@126.com
  • 作者简介:周遇巧(1995-),女,河南洛阳人,在读硕士研究生,研究方向:植物资源保护。E-mail: 2664975449@qq.com
  • 基金资助:
    河南省科技厅科技攻关项目(182102110329);南阳师范学院人才引进科研启动基金专项(18005);南阳师范学院大学生实践教学活动创新项目(SPCP2019257,SPCP2019271)

Study on Genetic Diversity of Actinidia Germplasm Resources Based on SCoT Markers

ZHOU Yuqiao1,GUO Guoye1,ZHOU Suo1,DU Ge2,LI Shulin2   

  1. (1.College of Life Science and Technology,Nanyang Normal University,Nanyang 473061,China; 2.Xixia Institute of Kiwifruit of Henan Province,Xixia 474573,China)
  • Received:2019-12-20 Published:2020-06-15 Online:2020-06-15

摘要: 利用SCoT分子标记对28份猕猴桃种质资源进行遗传多样性和UPGMA聚类关系研究。结果表明,8条引物对供试猕猴桃材料显示多态性,共扩增出75条清晰的条带,平均每条引物扩增9.1个多态性条带,多态性比率为97.3%。遗传相似系数在0.47~0.86,显示供试的猕猴桃种质资源具有丰富的遗传多样性。UPGMA聚类分析显示,在遗传相似系数0.64处可将28份猕猴桃种质划分为3个明显的聚类分支,部分中华猕猴桃、2个软枣猕猴桃与多数美味猕猴桃种质聚类,显示中华猕猴桃、软枣猕猴桃与美味猕猴桃具有较高的遗传相似性,种质亲缘关系较近;部分中华猕猴桃种质遗传变异较高,并与大籽猕猴桃有较近的遗传关系。

关键词: 猕猴桃, SCoT, 遗传多样性, 聚类分析

Abstract: SCoT molecular markers were applied to 28 Actinidia germplasm resources to study genetic diversity and UPGMA clustering relationship.The results showed that,8 polymorphic SCoT primers were selected,a total of 75 clear bands were obtained from 28 Actinidia materials with 8 primers,and 9.1 polymorphic bands were obtained from each primer on average,with a polymorphic ratio of 97.3%.The genetic similarity coefficient ranged from 0.47 to 0.86,indicating that the tested Actinidia germplasm resources had abundant genetic diversity.UPGMA clustering analysis showed that 28 Actinidia germplasms could be divided into three distinct clustering groups at the genetic similarity coefficient of 0.64,and part of A.chinensis,two A.arguta germplasms and most germplasms of A.chinensis var.deliciosa clustered together,which showed that A.chinensis, A.arguta and A.chinensis var.deliciosa had high genetic similarity and closely genetic relationship among these germplasms.The genetic variation of some A.chinensis germplasms was high and had closely genetic relationship with A.macrosperma.

Key words: Actinidia, SCoT, Genetic diversity, Clustering analysis

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