关键词: 三穗鸭, ORAI1基因, SNP, 蛋白质功能预测, 蛋壳品质

Abstract: In order to investigate the correlation between ORAI1 gene and eggshell quality of Sansui duck,a DNA mixing pool was constructed with Sansui duck as the research object.The ORAI1 gene of Sansui duck was detected for single nucleotide polymorphism(SNP) using PCR amplification and direct sequencing,which had significant effect on eggshell quality of Sansui duck.At the same time,the function of ORAI1 protein in Sansui duck was predicted.The results showed that ORAI1 gene of Sansui duck encoded 263 amino acids,which constituted an unstable hydrophilic protein.The secondary structure of ORAI1 protein consisted mainly of α helix,random curl,extension chain and β trun.After PCR amplification,three SNPs were found in the amplified segment,namely T84C,C188T and A471G.Synonymous mutation did not cause the change of amino acid,but caused the change of mRNA secondary structure and free energy of the gene,and the allele frequencies of each mutation site were different before and after mutation.The results indicated that there were three SNPs in ORAI1 gene,which could be used as a reference for molecular marker sites to improve eggshell quality.

Key words: Sansui duck, ORAI1 gene, SNP, Protein function prediction, Eggshell quality