河南农业科学 ›› 2020, Vol. 49 ›› Issue (4): 147-152.DOI: 10.15933/j.cnki.1004-3268.2020.04.021

• 畜牧·兽医 • 上一篇    下一篇

鹌鹑MyoG基因多态性与早期生长性能的关联分析

白俊艳1,2,时坤鹏1,卢小宁1,杨帅1,陈梦柯1,马永康1,巩慧荣1,董智豪1庞有志1,2,雷莹1,2,曹慧1,2,安肖凯1,常世增1,侯伟晓1,付学言1,樊红灯1,曹恒1   

  1. (1.河南科技大学 动物科技学院,河南 洛阳 471023; 2.洛阳市动物遗传育种重点实验室,河南 洛阳 471023)
  • 收稿日期:2019-10-08 出版日期:2020-04-15 发布日期:2020-04-15
  • 作者简介:白俊艳(1975-),女,内蒙古赤峰人,副教授,博士,主要从事动物分子遗传育种研究。E-mail:junyanbai@163.com
  • 基金资助:
    国家自然科学基金项目(31201777);河南省产学研合作项目(152107000095.0)

Association Analysis between MyoG Gene Polymorphism and Early Growth Performance of Quail

BAI Junyan1,2,SHI Kunpeng1,LU Xiaoning1,YANG Shuai1,CHEN Mengke1,MA Yongkang1,GONG Huirong1,DONG Zhihao1,PANG Youzhi1,2,LEI Ying1,2,CAO Hui1,2,AN Xiaokai1,CHANG Shizeng1,HOU Weixiao1,FU Xueyan1,FAN Hongdeng1,CAO Heng1   

  1. (1.College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471023,China;2.Luoyang Key Laboratory of Animal Genetics and Breeding,Luoyang 471023,China)
  • Received:2019-10-08 Published:2020-04-15 Online:2020-04-15

摘要: 为了寻找蛋用鹌鹑早期生长性能的分子标记,利用PCR-SSCP检测肌细胞生成素(MyoG)基因5′调控区A和B位点在中国黄羽鹌鹑、北京白羽鹌鹑、朝鲜鹌鹑群体中的多态性,并对MyoG基因5′调控区与鹌鹑的早期生长性能进行了关联性分析。结果表明, MyoG基因5′调控区A位点在蛋用鹌鹑中检测到AA、BB、CC、AB、AC、BC等6种基因型。MyoG基因5′调控区A位点的不同基因型与蛋用鹌鹑的体质量、胫长、胸宽、胸深、胸骨长、体长存在关联性(P<0.05)。MyoG基因5′调控区B位点在蛋用鹌鹑中检测到AA、BB、CC、DD、EE、AB、AC、AD、AE等9种基因型,不同基因型与蛋用鹌鹑的体质量、胫长、胸宽、胸深、胸骨长、体长存在关联性(P<0.05)。综上, MyoG基因5′调控区A、B位点对蛋用鹌鹑的早期生长性能有一定影响,可以作为蛋用鹌鹑早期生长性能的分子标记。

关键词: 蛋用鹌鹑, MyoG基因, SNP位点, 生长性能, 关联分析, 基因多态性

Abstract: In order to find molecular markers for early growth performance of egg quails,PCR-SSCP was used to detect the polymorphism of MyoG gene 5′ regulatory region A and B loci in Chinese yellow,Beijing white and Korean quails,and the relationship between the 5′ regulatory region of MyoG gene and early growth and development performance of quails was also analyzed.The results showed that 6 genotypes such as AA,BB,CC,AB,AC and BC were detected in the 5′ regulatory region A locus of MyoG gene in egg quails.Different genotypes of MyoG gene 5′ regulatory region A locus were associated with body weight,tibia length,chest width,chest depth,sternum length,body length of egg quails (P<0.05).Nine genotypes such as AA,BB,CC,DD,EE,AB,AC,AD and AE were detected in egg quails at site B of 5′regulatory region of MyoG gene.Different genotypes were correlated with body weight,tibia length,chest width,chest depth,sternum length and body length of egg quail (P<0.05).In conclusion,the A and B loci in the 5′ regulatory region of MyoG gene have some effects on the early growth performance of egg quails.The A and B loci in the 5′ regulatory region of MyoG gene can be used as molecular markers for the early growth performance of egg quails.

Key words: Egg quail; MyoG gene, SNP locus, Growth performance, Association analysis, Gene polymorphism

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