河南农业科学 ›› 2020, Vol. 49 ›› Issue (4): 93-100.DOI: 10.15933/j.cnki.1004-3268.2020.04.013

• 园艺 • 上一篇    下一篇

大白菜BrROP基因家族的生物信息学分析

闫敏,王晗,刘少华,顾小敏,许晔   

  1. (江苏第二师范学院 生命科学与化学化工学院,江苏 南京 211200)
  • 收稿日期:2019-12-10 出版日期:2020-04-15 发布日期:2020-04-15
  • 作者简介:闫敏(1988-),女,安徽六安人,实验师,博士,主要从事植物分子生物学研究。E-mail:yingying880506@163.com
  • 基金资助:
    江苏省高等学校自然科学基金项目(18KJB180002);江苏省自然科学基金项目(BK20170756)

Bioinformatics Analysis of BrROP Gene Family in Chinese Cabbage

YAN Min,WANG Han,LIU Shaohua,GU Xiaomin,XU Ye   

  1. (School of Life Sciences,Chemistry&Chemical Engineering,Jiangsu Second Normal University,Nanjing 211200,China)
  • Received:2019-12-10 Published:2020-04-15 Online:2020-04-15

摘要:  为了揭示大白菜BrROP基因家族的功能和进化关系,利用生物信息学方法对大白菜BrROP基因家族成员进行了鉴定,并对其基因结构、蛋白质序列、染色体定位、保守结构域、进化关系和表达模式等进行系统分析。结果表明,在大白菜基因组中共鉴定出22BrROP基因成员,在大白菜染色体上呈不均匀分布;氨基酸序列分析表明,BrROP蛋白的结构较保守,均含有G结构域(G1G5)、效应因子结合点、插入序列和C端可变区;根据系统发育进行聚类分析,大白菜BrROP基因可被分为4类,GroupⅠ、GroupⅡ、GroupⅢ和GroupⅣ;利用EMBL-EBI数据库对BrROP基因家族进行分析,22BrROP基因在大白菜根、茎、叶、花和角果中呈组织差异表达。

关键词: 大白菜, BrROP基因家族, 生物信息学, 系统分析

Abstract: To reveal the function and phylogenetic relationship of the BrROP gene family in Chinese cabbage,bioinformatics methods were used to identify the BrROP gene family members of Chinese cabbage, and the gene structure, protein sequence, chromosomal distribution, conserved domain,phylogenetic relationship and expression pattern were systematically analyzed.The results showed that 22 BrROP genes were identified in the Chinese cabbage genome,which were unevenly distributed on the chromosome of Chinese cabbage.Amino acid sequence analysis indicated that the structure of BrROP protein was conserved,including G domain(G1-G5),effector binding site,Rho insert region and Cterminal hypervariable region.According to the phylogenetic analysis,BrROP genes were divided into four categories,GroupⅠ,GroupⅡ,Group Ⅲ and Group Ⅳ.The expression of BrROP genes was analyzed using the EMBL-EBI database.22 BrROP genes were differentially expressed in root,stem,leaf,flower and silique.

Key words: Chinese cabbage, BrROP gene family, Bioinformatics, Phylogenetic analysis

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