河南农业科学 ›› 2020, Vol. 49 ›› Issue (4): 87-92.DOI: 10.15933/j.cnki.1004-3268.2020.04.012

• 植物保护 • 上一篇    下一篇

烟草病原菌及其生防菌株对烯酰吗啉的敏感性

郭敬1,汤玲玲1,赵世民2,李淑君3,康业斌1   

  1. (1.河南科技大学 林学院,河南 洛阳 471023;2.河南省洛阳市烟草公司,河南 洛阳 471023;3.河南省农业科学院 烟草研究所,河南 许昌 461000)
  • 收稿日期:2020-01-10 出版日期:2020-04-15 发布日期:2020-04-15
  • 通讯作者: 康业斌(1964-),男,河南南阳人,教授,博士,主要从事植物免疫学研究。E-mail:kangyb999@163.com
  • 作者简介:郭敬(1996-),女,河南新乡人,在读硕士研究生,研究方向:植物免疫学。E-mail:gjing2019@163.com
  • 基金资助:
    河南省烟草公司科学研究与技术开发重点项目(HYKJ201610,LYKJ201804)

Sensitivity of Tobacco Pathogens and Their Biocontrol Strains to Dimethomorph

GUO Jing1,TANG Lingling1,ZHAO Shimin2,LI Shujun3,KANG Yebin1   

  1. (1.College of Forestry,Henan University of Science and Technology,Luoyang 471023,China;2.Luoyang Branch of Henan Provincial Tobacco Company,Luoyang 471023,China;3.Tabocco Research Institute,Henan Academy of Agricultural Sciences,Xuchang 461000,China)
  • Received:2020-01-10 Published:2020-04-15 Online:2020-04-15

摘要: 为明确烟草疫霉(Phytophthora nicotianae)等主要土传病原真菌与哈茨木霉(Trichoderma harzianum)等供试生防菌株对烯酰吗啉的敏感性,采用菌丝生长速率法测定了烯酰吗啉对烟草疫霉、瓜果腐霉(Pythium aphanidermatum)、立枯丝核菌(Rhizoctonia solani)、茄病镰刀菌(Fusarium solani)、哈茨木霉菌丝生长的抑制作用,采用孢子抑制法测定了烯酰吗啉对烟草疫霉孢子囊形成、哈茨木霉分生孢子分化的抑制作用,采用稀释涂布平板法分别测定了烯酰吗啉对枯草芽孢杆菌(Bacillus subtilis)、金黄垂直链霉菌(Streptomyces aureoverticillatus)的抑制作用。结果表明,烯酰吗啉对烟草疫霉菌丝生长及孢子囊形成有显著抑制作用,对烟草疫霉菌丝生长的EC50为0.467 6 μg/mL;对哈茨木霉菌丝生长及分生孢子分化抑制作用较低;对瓜果腐霉、立枯丝核菌、茄病镰刀菌和枯草芽孢杆菌、金黄垂直链霉菌无明显抑制作用。因此,烯酰吗啉可以与供试生防菌株混合施用防治烟草黑胫病。

关键词: 烯酰吗啉;烟草, 病原菌;生防菌株;敏感性, 烟草黑胫病

Abstract: To identify the sensitivity of the main soil-borne pathogens of tobacco such as Phytophthora nicotianae and their candidate biocontrol strains such as Trichoderma harzianum to dimenthomorph,mycelium growth rate method,spore inhibition method,dilution-plate method were used to determine the inhibitory effect of dimethomorph on mycelium growth of P.nicotianae,Pythium aphanidermatum,Rhizoctonia solani,Fusarium solani,T.harzianum,sporangia production of P.nicotianae and conidia differentiation of T.harzianum,and on Bacillus subtilis,Streptomyces aureoverticillatus.The results showed that dimethomorph had a significant inhibitory effect on mycelium and sporangia of P.nicotianae,and the EC50 value of its mycelium growth was 0.467 6 μg/mL;and it had no strong effect on P.aphanidermatum,R.solani,F.solani,T.harzianum,B.subtilis,S.aureoverticillatus.Therefore,dimethomorph can be mixed with the candidate biocontrol strains to control tobacco black shank.

Key words: Dimenthomorph, Tobacco, Pathogen, Biocontrol strain, Sensitivity, Tobacco black shank

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