河南农业科学 ›› 2020, Vol. 49 ›› Issue (1): 157-164.DOI: 10.15933/j.cnki.1004-3268.2020.01.022

• 畜牧·兽医 • 上一篇    下一篇

维生素C对氨氮胁迫下大鳞鲃幼鱼存活及鳃抗氧化酶活性的影响

梁俊平1,张静1,覃宝利2,王宣朋2,蔺玉华2,丁辰龙1,2,吴学军2
  

  1. 1.河南师范大学 水产学院/河南省水产动物养殖工程技术研究中心,河南 新乡 453007;2.江苏省农业科学院 宿迁农科所,江苏宿迁 223800)
  • 收稿日期:2019-08-12 出版日期:2020-01-15 发布日期:2020-01-15
  • 通讯作者: 吴学军(1970-),男,江苏沭阳人,研究员级高级工程师,硕士,主要从事水产养殖研究。E-mail:271499738@qq.com 丁辰龙(1977-),男,江苏泗阳人,副研究员,硕士,主要从事特种水产养殖研究。E-mail:dcl0625@126.com
  • 作者简介:梁俊平(1981-),男,河北尚义人,副教授,博士,主要从事水产动物健康养殖研究。E-mail:liangjunpinglover@163.com
  • 基金资助:
    江苏省水产三新工程项目(Y2016-15);江苏省农业科技自主创新项目[CX(14)2076];宿迁市自主创新项目(SQCX2016-04,L201611);河南师范大学水产学院研究生科研创新项目(SC20180001)

Effects of Vitamin C on the Survival and Antioxidant Enzyme Activities of Juvenile Barbus capito under Ammonia-N Stress

LIANG Junping1,ZHANG Jing1,QIN Baoli2,WANG Xuanpeng2,LIN Yuhua2,DING Chenlong1,2,WU Xuejun2   

  1. (1. College of Fisheries,Henan Normal University/Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation,Xinxiang 453007,China; 2.Suqian Institute of Agricultural Sciences,Jiangsu Academy of Agricultural Sciences,Suqian 223800,China)
  • Received:2019-08-12 Published:2020-01-15 Online:2020-01-15

摘要: 为探究维生素C(Vc)对大鳞鲃幼鱼抗氨氮胁迫能力的影响,在水温(23.50±0.41)℃、pH值8.03±0.07条件下,选取体质量(3.71±0.60)g的大鳞鲃幼鱼,测定了氨氮对大鳞鲃幼鱼的24、48、72、96 h 半致死质量浓度,分析了水体添加Vc对大鳞鲃幼鱼死亡率以及鳃抗氧化酶活性的影响。结果显示,氨氮对大鳞鲃幼鱼的24、48、72、96 h 半致死质量浓度(LC50)分别为34.95、33.84、31.42、30.02 mg/L,安全质量浓度(SC)为3.00 mg/L。在96 h氨氮全致死质量浓度胁迫下,大鳞鲃幼鱼死亡率随水体Vc添加量增加呈显著下降趋势。在1/2的96 h LC50氨氮胁迫下(胁迫组),水体添加50 mg/L Vc后,大鳞鲃幼鱼超氧化物歧化酶(SOD)活性在24、96 h显著高于胁迫组(P<0.05),且与对照组无显著差异(P>0.05),过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-px)活性在48~96 h显著高于胁迫组(P<0.05);水体添加80 mg/L Vc后大鳞鲃幼鱼SOD活性在24~96 h显著高于胁迫组(P<0.05),且与对照组无显著差异(P>0.05),CAT和GSH-px活性在48~96 h均显著高于胁迫组(P<0.05),其中GSH-px活性在24~96 h与对照组无显著差异(P>0.05)。综上,氨氮胁迫下,大鳞鲃幼鱼的死亡率随氨氮质量浓度增加和胁迫时间延长呈线性增加,水体添加Vc可提高大鳞鲃幼鱼鳃抗氧化酶活性,增强其耐氨氮胁迫能力,降低大鳞鲃幼鱼死亡率。

关键词: 大鳞鲃, 氨氮毒性, 维生素C, 鳃, 死亡率, 抗氧化酶活性

Abstract: In order to reveal the effects of vitamin C(Vc) on anti-ammonia-N stress ability of juvenile Barbus capito,the median lethal concentrations of total ammonia-N were calculated for 24,48,72 and 96 h,and the effects of Vc on mortality and activities of antioxidant enzymes in gills were analyzed for juvenile Barbus capito under ammonia-N stress at water temperature of (23.50±0.41)℃ and pH of 8.03±0. 07.The results showed that,the median lethal concentrations of total ammonia-N were found to be 34.95 mg/L for 24 h,33.84 mg/L for 48 h,31.42 mg/L for 72 h,and 30.02 mg/L for 96 h to juvenile B.capito.The safe concentration of total ammonia-N was estimated to be 3. 00 mg/L for juvenile B.capito.The mortalities of juvenile B. capito were decreased with the rising of Vc concentration,when it was exposed to the 96 h total lethal concentration of ammonia-N.When juvenile B.capito was exposed to 15.01 mg/L ammonia-N with 50 mg/L Vc added,the SOD activities of gill significantly increased compared with the stress group(P<0.05) at 24 h and 96 h,and there was no significant difference compared with control group(P>0.05),the CAT and GSH-px activities increased significantly compared with the stress group (P<0.05) at 48—96 h.When juvenile B.capito was exposed to 15.01 mg/L ammonia-N with 80 mg/L Vc added,the activity of SOD increased significantly compared with the stress group(P<0.05) at 24—96 h and was consistent with the control group,the activities of CAT and GSH-px increased significantly compared with the stress group(P<0.05) at 48—96 h.In conclusion,the mortality of juvenile B.capito increases gradually with the rising of ammonia-N concentration or exposed period.Vc can reduce the mortality of juvenile B.capito exposed to ammonia-N effectively by improving the activity of antioxidant enzyme in gill,and enhancing the tolerance of juveniles B.capito to ammonia-N toxicity.

Key words: Barbus capito, Ammonia-N toxicity, Vitamin C, Gill, Mortality, Antioxidant enzyme activities

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