玉米脱落酸受体ZmPYL9基因的克隆及功能探究

doi:10.15933/j.cnki.1004-3268.2020.01.003

河南农业科学 ›› 2020, Vol. 49 ›› Issue (1): 18-23.DOI: 10.15933/j.cnki.1004-3268.2020.01.003

玉米脱落酸受体ZmPYL9基因的克隆及功能探究

王延召，鲁晓民，魏良明，周波，黄保

河南省农业科学院粮食作物研究所，河南郑州 450002

收稿日期:2019-05-15 出版日期:2020-01-15 发布日期:2020-01-15
通讯作者: 黄保（1980-），男，辽宁辽阳人，助理研究员，硕士，主要从事科研管理。E-mail:huangbao910@126.com
作者简介:王延召（1978-），男，河南禹州人，副研究员，博士，主要从事玉米遗传育种研究。E-mail:yz9839@126.com
基金资助:
河南省重大科技专项（151100111100）

Cloning and Function Analysis of Maize Abscisic Acid Receptor ZmPYL9 Gene

WANG Yanzhao,LU Xiaomin,WEI Liangming,ZHOU Bo,HUANG Bao

(Cereal Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

Received:2019-05-15 Published:2020-01-15 Online:2020-01-15

摘要/Abstract

摘要： 脱落酸（ABA）参与植物发育过程以及调控多种非生物胁迫的适应性，PYL基因作为ABA受体，直接影响ABA的生物合成。为了探究玉米中PYL家族基因的功能，克隆了一个ZmPYL9基因，该基因编码197个氨基酸；物种同源蛋白质系统进化分析发现，ZmPYL9与高粱同源蛋白相似度甚高，且具有相同的保守基序；启动子顺式作用元件分析发现，ZmPYL9基因ATG上游2 000 bp区域含有多种激素应答相关的结合位点；ZmPYL9基因组织表达分析结果表明，该基因属于组成型表达，在胚乳中高度表达。ZmPYL9基因正向响应外源ABA诱导，恢复正常培养后，该基因表达量急速下降且低于CK；但是ZmPYL9基因受PEG和PEG+ABA负向诱导，且在PEG+ABA胁迫下该基因表达量高于PEG胁迫下，恢复正常培养后，2种胁迫下该基因的表达量均表现上升趋势。说明ZmPYL9基因响应干旱胁迫和ABA诱导，且ABA可以提高干旱胁迫下该基因的表达量。

关键词: 玉米, 脱落酸受体, 组成型表达, 干旱胁迫, ABA诱导

Abstract: Abscisic acid(ABA)participates in plant development and regulates the adaptability of various abiotic stresses.The PYL gene acts as an ABA receptor and directly affects the biosynthesis of ABA.In order to explore the function of the PYL family gene in maize,a ZmPYL9 gene was cloned,encoding 197 amino acids.Evolutionary analysis of homologous proteins revealed that ZmPYL9 was highly homologous to sorghum homologous proteins and had the same conserved motif. Analysis of the promoter cis-acting element revealed that the 2 000 bp upstream of the ATG of the ZmPYL9 gene contained multiple hormoneresponsive binding sites.The tissue expression analysis of ZmPYL9 gene indicated that the gene was constitutively expressed and highly expressed in endosperm.The positive response of ZmPYL9 gene was induced by exogenous ABA,and the expression of this gene decreased rapidly and was lower than CK level after returning to normal rehydration culture.The ZmPYL9 gene was negatively induced by PEG and PEG+ABA,and the gene expression was higher than PEG stress under PEG+ABA stress. However,after normal rehydration,the expression of this gene under both stresses showed an upward trend.These indicate that ZmPYL9 gene responds to drought stress and ABA induction,and ABA can increase the expression of this gene under drought stress.

Key words: Maize, Abscisic acid receptor, Constitutive expression, Drought stress, ABA induction

王延召, 鲁晓民, 魏良明, 周波, 黄保. 玉米脱落酸受体ZmPYL9基因的克隆及功能探究[J]. 河南农业科学, 2020, 49(1): 18-23.

WANG Yanzhao, LU Xiaomin, WEI Liangming, ZHOU Bo, HUANG Bao. Cloning and Function Analysis of Maize Abscisic Acid Receptor ZmPYL9 Gene[J]. Journal of Henan Agricultural Sciences, 2020, 49(1): 18-23.

参考文献

［1］ZHU J K.Salt and drought stress signal transduction in plants［J］.Annu Rev Plant Biol,2002,53(1):247-273.

［2］XIONG L.Regulation of abscisic acid biosynthesis［J］.Pant Physiology,2003,133(1):29-36.

［3］WILKINSON S,DAVIES W J.ABAbased chemical signalling:The coordination of responses to stress in plants［J］.Plant Cell & Environment,2010,25(2):195-210.

［4］RAZEM F A,KEREAMY A,ABRAMS S R,et al.The RNAbinding protein FCA is an abscisic acid receptor［J］.Nature,2006,439(7074):290-294.

［5］RISK J M,MACKNIGHT R C,DAY C L.FCA does not bind abscisic acid［J］.Nature,2008,456(7223):290-294.

［6］LIU X,YUE Y,LI B,et al.Comment on “A G protein coupled receptor is a plasma membrane receptor for the plant hormone abscisic acid” ［J］.Science,2007,315(5819):1712.

［7］RISK J M,DAY C L,MACKNIGHT R C.Reevaluation of abscisic acidbinding assays shows that Gproteincoupled receptor 2 does not bind abscisic acid［J］.Plant Physiology,2009,150(1):6-11.

［8］PANDEY S,NELSON D C,ASSMANN S M.Two novel GPCRtype G proteins are abscisic acid receptors in Arabidopsis［J］.Cell,2009,136(1):136-148.

［9］PARK S Y,FUNG P,NISHIMURA N,et al.Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins［J］.Science,2009,324(5930):1068-1071.

［10］MA Y,SZOSTKIEWICE I,KORTE A,et al.Regulators of PP2C phosphatase activity function as abscisic acid sensors［J］.Science,2009,324(5930):1064-1068.

［11］YIN P,FAN H,HAO Q,et al.Structural insights into the mechanism of abscisic acid signaling by PYL proteins［J］.Nature Structural & Molecular Biology,2009,16(12):12301236.

［12］HE Y,HAO Q,LI W Q,et al.Identification and characterization of ABA receptors in Oryza sativa［J］.PLoS One,2014,9(4):e95246.

［13］CHAI Y M,JIA H F,LI C L,et al.FaPYR1 is involved in strawberry fruit ripening［J］.Journal of Experimental Botany,2011,62(14):5079-5089.

［14］WANG Y,WANG Y,KAI W,et al.Transcriptional regulation of abscisic acid signal core components during cucumber seed germination and under Cu²⁺,Zn²⁺,NaCl and simulated acid rain stresses［J］.Plant Physiology & Biochemistry,2014,76(5):67-76.

［15］李鸿杰,王盈阁,张夏伟,等.玉米脱落酸受体基因家族的生物信息学分析［J］.核农学报,2014,29(9):1657-1667.

LI H J,WANG Y G,ZHANG X W,et al.Bioinformatic analysis for abscisic acid perceptor gene family in maize［J］.Journal of Nuclear Agricultural Sciences,2014,29(9):1657-1667.

［16］RUBIO S,ANTONI R,PARK S Y,et al.In vitro reconstitution of an abscisic acid signalling pathway［J］.Nature,2009,462(7273):660-664.

［17］KIM H,HWANG H,HONG J W,et al.A rice orthologue of the ABA receptor,OsPYL/RCAR5,is a positive regulator of the ABA signal transduction pathway in seed germination and early seedling growth［J］.Journal of Experimental Botany,2012,63(2):1013-1024.

［18］KIM H,LEE K,HWANG H,et al.Overexpression of PYL5 in rice enhances drought tolerance,inhibits growth,and modulates gene expression［J］.Journal of Experimental Botany,2014,65(2):453-464.

［19］XUE T,WANG D,ZHANG S,et al.Genomewide and expression analysis of protein phosphatase 2C in rice and Arabidopsis［J］.BMC Genomics,2008,9(1):550.

［20］GONZALEZ G M,PIZZIO G A,ANTONI R,et al.Arabidopsis,PYR/PYL/RCAR receptors play a major role in quantitative regulation of stomatal aperture and transcriptional response to abscisic acid［J］.The Plant Cell,2012,24(6):2483-2496.

［21］SAAVEDRA X,MODREGO A,RODRIGUEZ D,et al.The nuclear interactor PYL8/RCAR3 of Fagus sylvatica FsPP2C1 is a positive regulator of abscisic acid signaling in seeds and stress［J］.Plant Physiology,2010,152(1):133-150.

［22］PIZZIO G A,RODRIGUEZ L,ANTONI R,et al.The PYL4 A194T mutant uncovers a key role of PYR1LIKE4/PROTEIN PHOSPHATASE 2CA interaction for abscisic acid signaling and plant drought resistance［J］.Plant Physiology,2013,163(1):441-455.

［23］SANTIAGO J,DUPEUX F,ROUND A,et al.The abscisic acid receptor PYR1 in complex with abscisic acid［J］.Nature,2009,462(7273):665-668.

[1]	李川, 张盼盼, 张美微, 牛军, 穆蔚林, 郭涵潇, 乔江方. 玉米花粉高温胁迫相关miRNA 的筛选及其靶基因分析[J]. 河南农业科学, 2024, 53(2): 1-16.
[2]	王鹏, 李笃花, 张绍辉, 杨孟宁, 安红柳, 方守国, 邓清超, 郭灵芳, 章松柏. 玉米纹枯病菌中新减毒病毒RsHV4 的特征研究[J]. 河南农业科学, 2024, 53(2): 92-100.
[3]	曹丽茹, 梁小菡, 马晨晨, 叶飞宇, 庞芸芸, 李伟亚, 张新, 鲁晓民. 玉米逆境胁迫响应基因ZmTPR1 的表达和功能分析[J]. 河南农业科学, 2024, 53(1): 12-21.
[4]	杨明达, 张素瑜, 李帅, 郑东方, 杨慎骄, 关小康, 王同朝. 不同播种灌溉方式对夏玉米出苗、干物质积累及转运和产量的影响[J]. 河南农业科学, 2024, 53(1): 22-31.
[5]	谢世兴, 蒋超, 赵冰欣, 董晓旭, 栗慧, 卢海博, 赵海超, 刘松涛, 魏东, 黄智鸿. 冀西北寒旱区不同鲜食玉米品种秸秆饲用品质特性研究[J]. 河南农业科学, 2024, 53(1): 32-40.
[6]	谢小文, 张心悦, 付家旭, 邵京, 温鹏飞, 王同朝, 卫丽. 干旱-复水处理下玉米差异表达bHLH基因的鉴定与分析[J]. 河南农业科学, 2023, 52(9): 33-44.
[7]	鲁晓民, 郭书磊, 张新, 魏良明, 张前进, 曹丽茹, 刘海静, 邓亚洲, 张震, 王振华. 不同密度下玉米茎秆抗倒伏相关性状的产量构成分析[J]. 河南农业科学, 2023, 52(9): 44-55.
[8]	徐丽娜, 安治良, 陈士林, 张怀胜, 进茜宁, 王平喜. 种植密度对玉米茎秆强度和穗部性状的影响[J]. 河南农业科学, 2023, 52(8): 11-17.
[9]	信龙飞, 娄闯, 冀保毅, 刘红云, 张艳玲. 干旱胁迫对桔梗光合作用和生理特性的影响[J]. 河南农业科学, 2023, 52(8): 69-77.
[10]	范晓庆, 赵心月, 王钰文, 张靓瑶, 袁进成, 刘颖慧, 瓮巧云. 青贮玉米和饲用油菜间作对饲草作物产量和品质的影响[J]. 河南农业科学, 2023, 52(7): 40-51.
[11]	魏全全, 顾小凤, 芶久兰, 张萌, 饶勇, 肖华贵. 氮肥及有机物料配施对黄壤冬油菜-玉米轮作田土壤微生物群落的影响[J]. 河南农业科学, 2023, 52(6): 41-50.
[12]	朱教宁, 高莉, 张静璇, 李永平, 汤昀, 史向远, 王秀红. 不同沼液回流比对鸡粪和玉米秸秆混合厌氧发酵性能的影响分析[J]. 河南农业科学, 2023, 52(6): 70-80.
[13]	王雅坤, 李鸿萍, 徐真真, 刘天学. 高温干旱复合胁迫对玉米光合生理的影响[J]. 河南农业科学, 2023, 52(5): 17-23.
[14]	豆丹丹, 孙建军, 郭玉玺, 王德新, 郭新海, 丁超明. 基于DUS 测试性状的黄淮海地区玉米自交系的遗传多样性及群体结构分析[J]. 河南农业科学, 2023, 52(5): 24-32.
[15]	赵海军, 史佳晴, 王彬, 郭益洋, 胡小丽, 韩赞平. 150 份玉米自交系籽粒及其品质性状的综合评价[J]. 河南农业科学, 2023, 52(5): 33-39.

玉米脱落酸受体ZmPYL9基因的克隆及功能探究

Cloning and Function Analysis of Maize Abscisic Acid Receptor ZmPYL9 Gene

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics