玉米HD-Zip转录因子基因ZmHOX32的克隆及功能探究

doi:10.15933/j.cnki.1004-3268.2019.11.005

河南农业科学 ›› 2019, Vol. 48 ›› Issue (11): 34-39.DOI: 10.15933/j.cnki.1004-3268.2019.11.005

玉米HD-Zip转录因子基因ZmHOX32的克隆及功能探究

张新¹，曹丽茹¹，张军²，魏良明¹，张前进¹，魏昕¹，王振华¹，鲁晓民¹

（1.河南省农业科学院粮食作物研究所，河南郑州 450002； 2.河南省蚕业科学研究院，河南南阳 473000）

收稿日期:2019-04-10 出版日期:2019-11-15 发布日期:2019-11-15
通讯作者: 鲁晓民（1980-）,男，河南许昌人，副研究员，博士，主要从事玉米遗传育种研究。E-mail:luxiaomin2004@163.com
作者简介:张新（1968-），女，河南罗山人，研究员，本科，主要从事玉米遗传育种研究。E-mail:zh5733764@126.com
基金资助:
国家重点研发计划项目（2016YFD0101205-4）；国家玉米产业技术体系项目(CARS-02-08)

Cloning and Function Analysis of Maize HD-Zip Transcription Factor Gene ZmHOX32

ZHANG Xin¹，CAO Liru¹，ZHANG Jun²，WEI Liangming¹，ZHANG Qianjin¹，WEI Xin¹，WANG Zhenhua¹，LU Xiaomin¹

( 1.Cereal Crop Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 2.Henan Sericulture Institute,Nanyang 473000,China)

Received:2019-04-10 Published:2019-11-15 Online:2019-11-15

摘要/Abstract

摘要： 同源异型域-亮氨酸拉链蛋白（HD-Zip）可通过与其他蛋白质互作参与激素信号传导途径，进而调控植物对逆境胁迫的应答和生长发育等过程。为验证及探究HD-Zip蛋白对干旱胁迫和ABA诱导的响应模式，从玉米抗旱自选系郑D58M中克隆了1个HD-Zip转录因子基因，暂时命名为ZmHOX32。ZmHOX32蛋白包含856个氨基酸，属于亲水性蛋白质，定位于细胞核内。进化树和保守基序分析发现，ZmHOX32蛋白序列与谷子、胡桃木的同源蛋白序列高度相似且保守基序完全一致。ZmHOX32基因启动子序列含有响应激素、非生物胁迫及光刺激的结合元件。qRT-PCR结果显示，ZmHOX32基因属于组成型表达基因，在营养分生组织中高表达，且受干旱胁迫和外源ABA的正向诱导,表明ZmHOX32基因参与干旱胁迫和ABA信号传导途径。

关键词: 玉米, 同源异型域-亮氨酸拉链蛋白, 干旱胁迫, 外源ABA, 正向诱导

Abstract: The homeodomain-leucine zipper protein(HD-Zip) can participate in the hormone signaling pathway by interacting with other proteins,thereby regulating the stress response and growth and develop ment of plants.To verify and explore the response pattern of HD-Zip protein to drought stress and ABA in duction,a HD-Zip transcription factor gene was cloned from maize drought-resistant self-selection line Zheng D58M,which was temporarily named ZmHOX32.The ZmHOX32 protein consisted 856 amino acids and belonged to a hydrophilic protein,which was localized in the nucleus.Phylogenetic tree and conserved motif analysis showed that the ZmHOX32 protein sequence was highly homologous to Setaria italica and Panicum hallii protein sequences,and the conserved motif was completely identical.The ZmHOX32 gene promoter sequence contained binding elements that responded to hormones,abiotic stresses and light stim ulation.The results of qＲT-PCＲ showed that ZmHOX32 gene was a constitutively expressed gene,which was highly expressed in vegetative meristem and was positively induced by drought stress and exogenous ABA.These results indicate that ZmHOX32 gene is involved in drought stress and ABA signaling path way．

Key words: Zea mays, HD-Zip, Drought stress, Exogenous ABA, Positive induction

中图分类号:

S513

张新, 曹丽茹, 张军, 魏良明, 张前进, 魏昕, 王振华, 鲁晓民. 玉米HD-Zip转录因子基因ZmHOX32的克隆及功能探究[J]. 河南农业科学, 2019, 48(11): 34-39.

ZHANG Xin, CAO Liru, ZHANG Jun, WEI Liangming, ZHANG Qianjin, WEI Xin, WANG Zhenhua, LU Xiaomin. Cloning and Function Analysis of Maize HD-Zip Transcription Factor Gene ZmHOX32[J]. Journal of Henan Agricultural Sciences, 2019, 48(11): 34-39.

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玉米HD-Zip转录因子基因ZmHOX32的克隆及功能探究

Cloning and Function Analysis of Maize HD-Zip Transcription Factor Gene ZmHOX32

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