关键词: 苦瓜, SSR, SRAP, 聚类分析, 遗传多样性

Abstract: The object of this study is to further shorten the operation process of molecular marker techno logy in bitter gourd(Momordica charantia) and maximize its application in molecular marker assisted se lection breeding.In this paper，a tubular plant DNAout kit was used to fast extract genomic DNA from bit ter gourd seeds，then the genomic DNA of 10 bitter gourd materials were amplified by PCＲ with 11 pairs of SSＲ primers and 13 pairs of SＲAP primers，and the genetic diversity of materials was also analyzed.Results indicated that the established SSＲ and SＲAP rapid amplification reaction system could obtain clear，stable and rich polymorphic bands.A total of 195 bands were amplified by 11 pairs of SSＲ primers， of which 164 bands were polymorphic，with a polymorphic band ratio of 85.7%.UPMGA cluster analysis showed that the genetic similarity coefficients of 10 materials ranged from 0.533 to 0.845，with an average of 0.690.At the threshold of 0.69，the tested materials could be divided into two groups.A total of 231 bands were amplified by 13 pairs of SＲAP primers，of which 201 bands were polymorphic，and the poly morphic band rate was 87.5%.UPMGA cluster analysis showed that the genetic similarity coefficients ranged from 0.554 to 0.775，with an average of 0.662，and all materials could be divided into two groups at the threshold of 0.66.Since both markers can obtain abundant polymorphic bands，the molecular mark er methodology can be applied to the genetic diversity analysis of bitter gourd． Moreover，the classification results by combining the two molecular marker techniques are more convincing．

Key words: Bitter gourd(Momordica charantia L.) , SSＲ, SＲAP, Cluster analysis, Genetic diversity