貂源铜绿假单胞菌耐药性及生物学特性

河南农业科学 ›› 2019, Vol. 48 ›› Issue (5): 122-129.

貂源铜绿假单胞菌耐药性及生物学特性

张明亮^1,2,3，张淼丹⁴，闫新武⁵，孙长江²，顾敬敏²，崔子寅²，韩文瑜²

1.安阳工学院生物与食品工程学院，河南安阳 455000； 2.吉林大学动物医学学院，吉林长春 130062；3.河南省动物疫病防控与营养免疫院士工作站，河南安阳 455000； 4.洛阳职业技术学院，河南洛阳 471000；5.河南省孟津县动物卫生监督所，河南孟津 471100

收稿日期:2018-12-22 出版日期:2019-05-15 发布日期:2019-05-15
通讯作者: 韩文瑜（1955-），男，河北阜城人，教授，博士，主要从事兽医微生物学和免疫学研究。E-mail:hanwy@jlu.edu.cn
作者简介:张明亮（1985-），男，河南驻马店人，讲师，博士，主要从事病原微生物和分子免疫学研究。E-mail:mingliang90909@163.com。张淼丹为同等贡献作者
基金资助:
国家高技术研究发展计划项目(2011AA10A210）；国家自然科学基金青年科学基金项目(31802170)；安阳工学院博士科研启动基金项目(BSJ2016013）

Received:2018-12-22 Published:2019-05-15 Online:2019-05-15

摘要/Abstract

摘要： 为了解貂源铜绿假单胞菌流行株基本特性，对分离的20株铜绿假单胞菌进行药物敏感性试验、血清型鉴定、ToxA基因检测、强毒株筛选及绝对致死量(LD₁₀₀)测定。结果表明，铜绿假单胞菌分离株耐药现象比较严重，对氨苄西林、头孢呋辛、头孢曲松、头孢噻吩、头孢噻肟、头孢西丁、头孢唑林和四环素不敏感，对妥布霉素敏感率为95%；铜绿假单胞菌分离株的血清型为G型和B型，分别占85%和15%；20株铜绿假单胞菌均能检测出毒素基因ToxA；小鼠致死性试验表明，铜绿假单胞菌分离株之间毒力差异很大，少数菌株如ZHDL9、ZHDL6等均表现出较强的毒力。以菌株ZHDL9为代表株建立小鼠鼻腔感染模型，采用建立的感染模型测定得菌株ZHDL9对小鼠的LD₁₀₀为5×10⁷ cfu。由此可见，分离的20株铜绿假单胞菌对临床常用抗生素耐药严重，对妥布霉素的敏感率为95%，提示该种抗生素可用于临床治疗水貂出血性肺炎，而菌株ZHDL9可作为生物制品研发的候选菌株。

张明亮，张淼丹，闫新武，孙长江，顾敬敏，崔子寅，韩文瑜. 貂源铜绿假单胞菌耐药性及生物学特性[J]. 河南农业科学, 2019, 48(5): 122-129.

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