绵羊miR-1基因前体序列的多态性分析

摘要/Abstract

摘要： 为了检测不同产肉性能的绵羊群体中miR-1基因前体序列的单核苷酸多态性（Single nucleotide polymorphism，SNP），并与绵羊骨骼肌发育进行相关性分析，采用不对称聚合酶链式反应-单链构象多态性（PCR-SSCP）技术对萨福克羊和湖羊2种产肉性能不同绵羊的miR-1基因前体序列进行SNP检测，并预测该SNP位点对miR-1前体序列二级结构稳定性所造成的影响。结果表明，在绵羊miR-1基因前体成熟序列的下游115 bp处检测到1个A→G突变，并且该SNP位点与绵羊的产肉性能有一定的相关性；由国外引进的优良肉羊品种萨福克羊，GG基因型为其优势基因型，G等位基因频率为0.76；通过miR-1 基因前体序列二级结构预测发现，该SNP位点能够使其发生变化，使ΔG降低1.5 kJ/mol。综上所述，绵羊miR-1基因前体序列的SNP位点可能对miR-1前体的加工成熟产生重要影响，最终影响绵羊的产肉性能。

关键词: 绵羊, miR-1基因前体序列, 骨骼肌, 不对称PCR-SSCP, 多态性

张翔宇，吴玉宇，牛孙敏，崔玉龙，胡圣伟，倪伟. 绵羊miR-1基因前体序列的多态性分析[J]. 河南农业科学, 2018, 47(12): 121-125.

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