河南农业科学 ›› 2018, Vol. 47 ›› Issue (7): 1-7.
• 综述 • 下一篇
崔俊霞,魏康宁,谢伊源,王 丽,李用芳*
收稿日期:
2017-12-25
出版日期:
2018-07-15
发布日期:
2018-07-15
通讯作者:
李用芳(1966-),女,河南林州人,教授,博士,主要从事转录后调控与植物生长发育研究。E-mail:041031@htu.edu.cn
作者简介:
崔俊霞(1992-),女,河南周口人,在读硕士研究生,研究方向:生物学。E-mail:13422099632@qq.com
基金资助:
Received:
2017-12-25
Published:
2018-07-15
Online:
2018-07-15
摘要: MicroRNA(miRNA)是真核生物中具有重要调控功能的一类小分子RNA。植物miRNA通过碱基互补配对方式引导RNA沉默复合体识别靶基因并介导靶基因mRNA被剪切或蛋白质翻译被抑制,所以对植物miRNA靶基因的分析和验证是研究miRNA功能必不可少的一部分。植物miRNA靶基因可通过生物信息学方法预测,主要根据 miRNA与 mRNA的互补程度进行,然而理论上预测的靶基因尚有待于进一步的试验验证。简要介绍了植物miRNA产生过程的最新研究进展以及靶基因的预测方法,重点综述了植物miRNA靶基因的验证方法,包括RLM-5′RACE、降解组测序、瞬时共表达和转基因系统,为更深入地研究植物miRNA介导的调控机制提供参考。
崔俊霞,魏康宁,谢伊源,王 丽,李用芳. 植物microRNA靶基因的预测与验证技术研究进展[J]. 河南农业科学, 2018, 47(7): 1-7.
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