鸭源鸡杆菌外膜蛋白A基因克隆及结构与功能分析

河南农业科学 ›› 2017, Vol. 46 ›› Issue (8): 147-151.

鸭源鸡杆菌外膜蛋白A基因克隆及结构与功能分析

王艳，王继洋，王坤芃，彭志锋，杨霞，王川庆^*

河南农业大学牧医工程学院,河南郑州 450002

收稿日期:2017-03-21 出版日期:2017-08-15 发布日期:2017-08-15
通讯作者: 王川庆（1954-），男，河南濮阳人，教授，博士，主要从事动物传染病发病机制及防制研究。E-mail：wchuanq@163.com
作者简介:王艳（1990-），女，河南信阳人，在读硕士研究生，研究方向：动物传染病发病机制及防制。E-mail：1078070117@qq.com
基金资助:
国家自然科学基金项目（3177130375）；河南省高校科技创新团队与支持计划资助项目（14IRTSHN015）

Received:2017-03-21 Published:2017-08-15 Online:2017-08-15

摘要/Abstract

摘要：

为筛选鸭源鸡杆菌（G.anatis）潜在的保护性抗原基因，参考GenBank中G.anatisUMN179的外膜蛋白A（OmpA）基因序列设计1对引物，对鸭源鸡杆菌PDS-RZ-1-SLG（RZ）株的OmpA基因进行克隆，并应用相关软件对其核苷酸及氨基酸序列进行生物信息学分析。结果显示，OmpA基因大小为1 083 bp，编码360个氨基酸；鸭源鸡杆菌RZ株的OmpA与UMN179、F149及12656/12的OmpA氨基酸相似性分别为93.2%、95.2%、92.7%；OmpA蛋白分子质量为38.4 ku，等电点为6.77，具有稳定性，N端有1个疏水性的α螺旋信号肽。

王艳，王继洋，王坤芃，彭志锋，杨霞，王川庆. 鸭源鸡杆菌外膜蛋白A基因克隆及结构与功能分析[J]. 河南农业科学, 2017, 46(8): 147-151.

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