拟南芥miR156与miR399在低磷胁迫中的对话机制初探

河南农业科学 ›› 2019, Vol. 48 ›› Issue (7): 54-58.

拟南芥miR156与miR399在低磷胁迫中的对话机制初探

刘浩¹，李玉龙¹，汤萌萌¹，雷凯健²

(1.河南大学生命科学学院/棉花生物学国家重点实验室，河南开封 475004； 2.河南大学药物研究所，河南开封 475004)

收稿日期:2019-02-10 出版日期:2019-07-15 发布日期:2019-07-15
通讯作者: 雷凯健(1981-),女，河南开封人，副教授,博士，主要从事植物逆境生物学研究。E-mail:leikaijian@126.com
作者简介:刘浩（1978-），男，河南许昌人，讲师，博士，主要从事植物激素信号互作研究。E-mail:1016736224@qq.com
基金资助:
国家自然科学基金项目（31601140）;河南省高等学校重点科研项目（16A180002，15A180001）

Received:2019-02-10 Published:2019-07-15 Online:2019-07-15

摘要/Abstract

摘要： 为了进一步研究miR156与miR399在低磷响应过程中是否存在对话机制，采用杂交方法，将35S:MIM156（miR156功能降低拟南芥植株）与 miR399f（miR399f超表达拟南芥植株）杂交获得杂交转基因植株35S:MIM156 miR399f。采用原位显色法和根际酸化能力测定法，研究低磷胁迫下35S:MIM156、miR399f及35S:MIM156 miR399f植株根际酸化情况，并测定其花青素和无机磷含量。结果表明，35S:MIM156植株的花青素含量较野生型（WT）植株显著降低，miR399f、35S:MIM156 miR399f植株与WT无显著差异；35S:MIM156植株的酸化能力较WT显著降低，miR399f、35S:MIM156 miR399f植株酸化能力均较WT显著增强；35S:MIM156、miR399f、35S:MIM156 miR399f植株的无机磷含量均较WT显著升高。综合分析，推测在低磷条件下miR399参与了miR156调控的花青素积累和根际酸化过程，miR156和 miR399共同参与磷平衡过程。

关键词: miR399, miR156, 低磷胁迫, 无机磷含量, 根际酸化

刘浩，李玉龙，汤萌萌，雷凯健. 拟南芥miR156与miR399在低磷胁迫中的对话机制初探[J]. 河南农业科学, 2019, 48(7): 54-58.

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